Part:BBa_K1088009:Design

B. subtilis dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG ind

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2505
  Illegal EcoRI site found at 3162
  Illegal PstI site found at 2563
  Illegal PstI site found at 3009
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2505
  Illegal EcoRI site found at 3162
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 2563
  Illegal PstI site found at 3009
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2505
  Illegal EcoRI site found at 3162
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2505
  Illegal EcoRI site found at 3162
  Illegal PstI site found at 2563
  Illegal PstI site found at 3009
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2505
  Illegal EcoRI site found at 3162
  Illegal PstI site found at 2563
  Illegal PstI site found at 3009
  Illegal NgoMIV site found at 2462
  Illegal AgeI site found at 2355
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2304
  Illegal BsaI.rc site found at 4018
  Illegal SapI.rc site found at 3003
  

Design Notes

The terminator aswell as the RBS and promoter was part of the reverse primer and forward primer for the LacI part, respectively. However we did insert a scar sequence between the RBS and startcodon to improve efficiency. There is also scar sites between the terminator (BBa_10002) and the rest of this composite part (BBa_K1088008). In the BBa_K1088008 part there is specific sites for scars aswell. Go to that parts page to investigate further.

Source

With the exception of the artificial terminator and the GFP part from BBa_K1088008 (GFP = BBa_E0040), everything derives from E. coli K-12 MG1655